Ochratoxin A-Imprinted nanoMIPs Prepared by Solid Phase Synthesis: Effect of Mimic Template on Binding Properties

The solid-phase polymerization synthesis (SPPS) represents one of the most innovative approaches to preparation nano-sized molecularly imprinted polymers. One its main features consists use a solid support on which template molecule is covalently grafted. It implies that imprinting process does not involve target as is, but, rather, structural modification it. known rationally designed mimic N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanine (CHNA-Phe) able generate, by bulk polymerization, materials capable recognizing mycotoxin Ochratoxin A (OTA). In this work, we wanted verify whether CHNA-Phe can be useful in SPPS technique. binding isotherm were measured pH range 4–8 and affinities for OTA compared, showing CHNA-Phe-imprinted nanoMIPs recognize, buffered water, equally well OTA, overall molecular recognition depends markedly from pH-related ionic interactions between ligand site. There results confirm method, it possible convenient templates whose three-dimensional structure some extent different without substantial loss selectivity or affinity.